The use of histological analysis for the detection of somatic embryos in sugarcane

The aim of this study was to establish an in vitro system for the induction, maturation and regeneration of somatic embryo in sugarcane from buds of cultivar RB 867515. Embryogenic calluses were obtained on semi-solid MS medium supplemented with 4.42 mg L-1 2,4-D. After four weeks of culture of explants on the callus induction medium, globular structures were obtained. At the end of 20 days in maturation medium, somatic embryos were observed. Histological analysis showed somatic embryos with caulinarand root apex, protodermal tissue, and the vascular system, which  apparently has no connection with the vascular tissue explant that gave rise to it confirming the presence of the somatic embryo. The embryos were transferred to regeneration medium containing 1 mg L-1 GA3 and BAP, and after 1 to 2 weeks of culture, green points were observed, indicating the beginning of the formation of shoots. Key words: Saccharum spp, bud culture, 2.4-D, morphogenetic pathway, embryogenesis, plant regeneration

A APOSENTADORIA ESPECIAL DO SERVIDOR PÚBLICO E SUAS POLÊMICAS

The article brings considerations about excitement about the special retirement of public agents. This is because, as far as social security is concerned, the Federal Constitution guaranteed to the public agente the right to the referred benefit, leaving, however, to the charge of the Complementary Law the regulation of this matter, and until the present moment there was not the creation of such law , thus generating several doubts regarding the applicability of the programmatic norm provided for in our Political Charter. Because of this, over time, there were many legal changes, normative acts issued by the Ministry of Social Security and Labor, around the subject. Many injunctions were filed with the Superior Court of Justice, and only then, with the issue of Supreme Court Precedent 33, which states that "The rules of the general social security system apply to the public agent, as appropriate. on special retirement referred to in article 40, paragraph 4, item III of the Federal Constitution, until the issue of a specific supplementary law "was given to civil servants the minimum legal certainty with respect to judicial decisions on special retirement.O artigo traz considerações sobre celeuma acerca da aposentadoria especial dos servidores públicos. Isto porque, no que tange a previdência social, a Constituição Federal garantiu ao servidor público o direito ao benefí­cio referido, deixando, contudo, ao encargo da Lei Complementar a regulamentação desta matéria, sendo que até o presente momento não houve a criação de tal lei, gerando assim várias dúvidas no que diz respeito a aplicabilidade da norma programática prevista na nossa Carta Polí­tica. Em virtude disso, ao longo do tempo, ocorreram muitas alterações legais, atos normativos expedidos pelo Ministério da Previdência Social e do Trabalho, em torno do tema. Muitos mandados de injunção foram impetrados junto ao Superior Tribunal de Justiça, e só então, com a edição da Súmula 33 do STF, a qual dispõe que "Aplicam-se ao servidor público, no que couber, as regras do regime geral da previdência social sobre aposentadoria especial de que trata o artigo 40, § 4º, inciso III da Constituição Federal, até a edição de lei complementar especí­fica" foi dado ao servidores públicos o mí­nimo de segurança jurí­dica no que diz respeito í s decisões judiciais acerca da aposentadoria especial

Characteristics and outcome of patients with newly diagnosed advanced or metastatic lung cancer admitted to intensive care units (ICUs)

BACKGROUND: Although patients with advanced or metastatic lung cancer have poor prognosis, admission to the ICU for management of life-threatening complications has increased over the years. Patients with newly diagnosed lung cancer appear as good candidates for ICU admission, but more robust information to assist decisions is lacking. The aim of our study was to evaluate the prognosis of newly diagnosed unresectable lung cancer patients. METHODS: A retrospective multicentric study analyzed the outcome of patients admitted to the ICU with a newly diagnosed lung cancer (diagnosis within the month) between 2010 and 2013. RESULTS: Out of the 100 patients, 30 had small cell lung cancer (SCLC) and 70 had non-small cell lung cancer. (Thirty patients had already been treated with oncologic treatments.) Mechanical ventilation (MV) was performed for 81 patients. Seventeen patients received emergency chemotherapy during their ICU stay. ICU, hospital, 3- and 6-month mortality were, respectively, 47, 60, 67 and 71%. Hospital mortality was 60% when invasive MV was used alone, 71% when MV and vasopressors were needed and 83% when MV, vasopressors and hemodialysis were required. In multivariate analysis, hospital mortality was associated with metastatic disease (OR 4.22 [1.4-12.4]; p = 0.008), need for invasive MV (OR 4.20 [1.11-16.2]; p = 0.030), while chemotherapy in ICU was associated with survival (OR 0.23, [0.07-0.81]; p = 0.020). CONCLUSION: This study shows that ICU management can be appropriate for selected newly diagnosed patients with advanced lung cancer, and chemotherapy might improve outcome for patients with SCLC admitted for cancer-related complications. Nevertheless, tumors' characteristics, numbers and types of organ dysfunction should be taken into account in the decisional process before admitting these patients in ICU.Peer reviewe

Detection of curved lines with B-COSFIRE filters: A case study on crack delineation

The detection of curvilinear structures is an important step for various computer vision applications, ranging from medical image analysis for segmentation of blood vessels, to remote sensing for the identification of roads and rivers, and to biometrics and robotics, among others. %The visual system of the brain has remarkable abilities to detect curvilinear structures in noisy images. This is a nontrivial task especially for the detection of thin or incomplete curvilinear structures surrounded with noise. We propose a general purpose curvilinear structure detector that uses the brain-inspired trainable B-COSFIRE filters. It consists of four main steps, namely nonlinear filtering with B-COSFIRE, thinning with non-maximum suppression, hysteresis thresholding and morphological closing. We demonstrate its effectiveness on a data set of noisy images with cracked pavements, where we achieve state-of-the-art results (F-measure=0.865). The proposed method can be employed in any computer vision methodology that requires the delineation of curvilinear and elongated structures.Comment: Accepted at Computer Analysis of Images and Patterns (CAIP) 201

Feminicida: la vigencia de la ley n ° 13.104 / 2015 sobre la lucha contra la violencia de género

O tema deste artigo é o feminicí­dio. Investigou-se o seguinte problema: A eficácia da Lei nº 13.104/2015 no combate í  violência do Gênero. Cogitou-se a seguinte hipótese: "A Lei nº 13.104, de 2015, que inseriu a qualificadora feminicí­dio no Código Penal Brasileiro, é um instrumento eficaz no combate í  violência contra a mulher?". O objetivo geral é analisar a eficácia da Lei nº 13.104/2015, no combate í  violência do gênero. Os objetivos especí­ficos são: investigar o contexto histórico da violência contra a mulher; examinar a qualificadora feminicí­dio e sua aplicação; discutir a eficácia da Lei como medida de proteção. Este trabalho é importante em uma perspectiva individual devido os seus consequentes reflexos para as mulheres; para a ciência, por analisar a eficácia que a lei como uma norma apresenta em seu ordenamento jurí­dico; agrega í  sociedade pelo fato de buscar realizar uma análise sobre o contexto histórico-jurí­dico e suas consequências. Trata-se de uma pesquisa qualitativa teórica com duração de 6 meses.The subject of this article is femicide. The following problem was investigated: The effectiveness of Law No. 13.104 / 2015 in combating Gender violence. The following hypothesis was considered: "Is Law No. 13.104 of 2015, which inserted the qualifying feminicide in the Brazilian Penal Code, an effective instrument to combat violence against women?". The general objective is to analyze the effectiveness of Law No. 13.104 / 2015 in combating gender violence. The specific objectives are: to investigate the historical context of violence against women; examine the qualifying feminicide and its application; discuss the effectiveness of the law as a protective measure. This work is important from an individual perspective because of its consequent consequences for women; for science, for analyzing the effectiveness that the law as a norm presents in its legal order; It adds to society because it seeks to perform an analysis of the historical-legal context and its consequences. This is a theoretical qualitative research lasting 6 months.El tema de este artí­culo es el feminicidio. Se investigó el siguiente problema: La efectividad de la Ley N ° 13.104 / 2015 en la lucha contra la violencia de género. Se consideró la siguiente hipótesis: "¿Es la Ley N ° 13.104 de 2015, que insertó el feminicidio calificado en el Código Penal brasileño, un instrumento eficaz para combatir la violencia contra las mujeres?". El objetivo general es analizar la efectividad de la Ley N ° 13.104 / 2015 en la lucha contra la violencia de género. Los objetivos especí­ficos son: investigar el contexto histórico de la violencia contra la mujer; examinar el feminicidio calificado y su aplicación; Discutir la efectividad de la ley como medida de protección. Este trabajo es importante desde una perspectiva individual debido a sus consecuentes consecuencias para las mujeres; para la ciencia, para analizar la efectividad que la ley como norma presenta en su orden legal; Se agrega a la sociedad porque busca realizar un análisis del contexto histórico-legal y sus consecuencias. Esta es una investigación cualitativa teórica que dura 6 meses

Reflexiones de la insencia de trabajo inss

Objetiva-se com o presente artigo a análise dos reflexos provocados pela sentença trabalhista perante o órgão responsável pela previdência social, denominado de INSS. Isto porque, a eficácia de tal sentença tem gerado polêmicas na jurisprudência trabalhista brasileira, pois há uma resistência por parte dos órgãos administrativos previdenciários, no sentido de não reconhecer a sentença e o acordo judicial trabalhista como prova única do tempo de serviço para fins de concessão de benefí­cios. Todavia, após a EC nº 20 de 1998, houve uma ampliação da competência da Justiça do Trabalho para executar as contribuições previdenciárias decorrentes de suas decisões, tornando a partir daí­ um grande avanço de inegável valia e qualificação desta Justiça Especializada, pois além de resguardar os direitos dos trabalhadores, também serviu de meio fiscalizatório, e tão importante quanto, foram os montantes que se passou a arrecadar para os cofres da Autarquia Previdenciária vertidos da execução perante a Justiça do Trabalho, o que sem dúvidas auxilia sobremaneira o sustento da Seguridade Social. Posteriormente, o entendimento jurisprudencial foi se consolidando nesse sentido, sobretudo com o advento dos enunciados das súmulas nº 31 e 34 da TNU (Turma Nacional de Uniformização de jurisprudência), as quais consideram a anotação na CTPS decorrente de sentença trabalhista homologatória prova constitutiva para fins previdenciários", cujo iní­cio deve ser contemporâneo í  época dos fatos a provar".The objective of this article is to analyze the reflexes provoked by the labor sentence before the social security body, called INSS. This is because, the effectiveness of such a sentence has generated controversy in the Brazilian labor jurisprudence, because there is a resistance on the part of the social security administrative bodies, in the sense of not recognizing the sentence and the labor judicial agreement as a single proof of the time of service for concession purposes. of benefits. However, after EC No. 20 of 1998, there was an extension of the jurisdiction of the Labor Court to execute the social security contributions resulting from its decisions, making from then on a great advance of undeniable value and qualification of this Specialized Justice, because besides safeguarding the workers' rights, also served as a means of enforcement, and just as important, were the amounts that began to be collected for the coffers of the Social Security Authority poured from execution before the Labor Court, which undoubtedly helps the support of Social Security. Subsequently, the jurisprudential understanding was consolidated in this sense, especially with the advent of the statements of the summaries 31 and 34 of the TNU (National Classification of Uniformization of jurisprudence), which consider the annotation in CTPS resulting from homologation labor judgment constitutive proof for purposes social security ", whose beginning must be contemporary to the time of the facts to prove".El objetivo de este artí­culo es analizar los reflejos provocados por la sentencia laboral ante el organismo de seguridad social, llamado INSS. Esto se debe a que la efectividad de dicha sentencia ha generado controversia en la jurisprudencia laboral brasileña, porque existe una resistencia por parte de los órganos administrativos de la seguridad social, en el sentido de no reconocer la sentencia y el acuerdo judicial laboral como una prueba única de la duración del servicio para fines de concesión. de beneficios. Sin embargo, después de la CE No. 20 de 1998, hubo una extensión de la jurisdicción de la Corte Laboral para ejecutar las contribuciones a la seguridad social derivadas de sus decisiones, haciendo desde entonces un gran avance de valor innegable y calificación de esta Justicia Especializada, porque además de salvaguardar el Los derechos de los trabajadores, también sirvieron como un medio para hacer cumplir, e igual de importante, fueron los montos que comenzaron a recaudarse para las arcas de la Autoridad de Seguridad Social vertidas de la ejecución ante el Tribunal Laboral, lo que sin duda ayuda al apoyo de la Seguridad Social. Posteriormente, la comprensión jurisprudencial se consolidó en este sentido, especialmente con el advenimiento de las declaraciones de los resúmenes 31 y 34 de la TNU (Clasificación Nacional de Uniformización de Jurisprudencia), que consideran la anotación en CTPS como resultado de la homologación de la prueba de juicio laboral para propósitos seguridad social ", cuyo comienzo debe ser contemporáneo a la época de los hechos a probar"

A large-scale study of a poultry trading network in Bangladesh: implications for control and surveillance of avian influenza viruses

Since its first report in 2007, avian influenza (AI) has been endemic in Bangladesh. While live poultry marketing is widespread throughout the country and known to influence AI dissemination and persistence, trading patterns have not been described. The aim of this study is to assess poultry trading practices and features of the poultry trading networks which could promote AI spread, and their potential implications for disease control and surveillance. Data on poultry trading practices was collected from 849 poultry traders during a cross-sectional survey in 138 live bird markets (LBMs) across 17 different districts of Bangladesh. The quantity and origins of traded poultry were assessed for each poultry type in surveyed LBMs. The network of contacts between farms and LBMs resulting from commercial movements of live poultry was constructed to assess its connectivity and to identify the key premises influencing it

Acute Pelvic Ischemia: a Fatal Outcome after Endovascular Aortoiliac Aneurysm Repair with Iliac Branch Device

Introdução: A oclusão da artéria hipogástrica pode ser necessária na reparação endovascular de aneurismas da aorta abdominal (EVAR). A oclusão intencional da hipogástrica pode ter complicações isquémicas. As endopróteses de bifurcação ilíaca (IBD) surgiram como alternativa endovascular à oclusão da hipogástrica em doentes com elevado risco para isquemia pélvica. Os autores descrevem um caso de oclusão precoce do ramo hipogástrico de IBD com graves consequências clínicas. Caso clínico: Sexo masculino, de 74 anos, com aneurisma da aorta abdominal (diâmetro máximo de 55 mm) com envolvimento de ambas as bifurcações ilíacas e segmentos proximais das hipogástricas (diâmetro máximo de 31 e 32 mm), submetido a EVAR com revascularização hipogástrica esquerda via IBD (Cook Zenith®) e coiling+overstenting da artéria hipogástrica contralateral. O procedimento decorreu sem complicações e a angiografia final mostrava permeabilidade da hipogástrica revascularizada e escassa colateralidade pélvica. O pós-operatório imediato complicou-se de dor lombar e glútea bilateral associada a manifestações cutâneas isquémicas e monoparesia do membro inferior esquerdo. Por agravamento progressivo nas primeiras 24h e angioTC com oclusão do stent da hipogástrica esquerda, procedeu-se novamente a revascularização da hipogástrica, com bom resultado na angiografia final. Apesar da revascularização bem-sucedida, houve agravamento progressivo do estado geral, com isquemia pélvica irreversível e rabdomiólise. Óbito ao 5.◦dia pós-operatório. Conclusão: A isquemia pélvica aguda é uma complicação grave e frequentemente fatal que pode advir da oclusão bilateral das artérias hipogástricas. A falência da revascularização por IBD pode ser fatal, pelo que os autores aconselham um cuidado redobrado no controlo angiográfico final e um baixo limiar para investigação na suspeita de complicações pós-operatórias. Se maior risco de falência técnica, embolização ou escassa colateralidade pélvica, a preservação bilateral de fluxo nas artérias hipogástricas pode estar recomendada

Complete mitochondrial DNA sequences provide new insights into the Polynesian motif and the peopling of Madagascar

More than a decade of mitochondrial DNA (mtDNA) studies have given the 'Polynesian motif' renowned status as a marker for tracing the late-Holocene expansion of Austronesian speaking populations. Despite considerable research on the Polynesian motif in Oceania, there has been little equivalent work on the western edge of its expansion - leaving major issues unresolved regarding the motif's evolutionary history. This has also led to considerable uncertainty regarding the settlement of Madagascar. In this study, we assess mtDNA variation in 266 individuals from three Malagasy ethnic groups: the Mikea, Vezo, and Merina. Complete mtDNA genome sequencing reveals a new variant of the Polynesian motif in Madagascar; two coding region mutations define a Malagasy-specific sub-branch. This newly defined 'Malagasy motif' occurs at high frequency in all three ethnic groups (13-50%), and its phylogenetic position, geographic distribution, and estimated age all support a recent origin, but without conclusively identifying a specific source region. Nevertheless, the haplotype's limited diversity, similar to those of other mtDNA haplogroups found in our Malagasy groups, best supports a small number of initial settlers arriving to Madagascar through the same migratory process. Finally, the discovery of this lineage provides a set of new polymorphic positions to help localize the Austronesian ancestors of the Malagasy, as well as uncover the origin and evolution of the Polynesian motif itself

HAGE (DDX43) is a biomarker for poor prognosis and a predictor of chemotherapy response in breast cancer

Background: HAGE protein is a known immunogenic cancer-specific antigen. Methods: The biological, prognostic and predictive values of HAGE expression was studied using immunohistochemistry in three cohorts of patients with BC (n=2147): early primary (EP-BC; n=1676); primary oestrogen receptor-negative (PER-BC; n=275) treated with adjuvant anthracycline-combination therapies (Adjuvant-ACT); and primary locally advanced disease (PLA-BC) who received neo-adjuvant anthracycline-combination therapies (Neo-adjuvant-ACT; n=196). The relationship between HAGE expression and the tumour-infiltrating lymphocytes (TILs) in matched prechemotherapy and postchemotherapy samples were investigated. Results: Eight percent of patients with EP-BC exhibited high HAGE expression (HAGEþ) and was associated with aggressive clinico-pathological features (Ps<0.01). Furthermore, HAGEþexpression was associated with poor prognosis in both univariate and multivariate analysis (Ps<0.001). Patients with HAGE+ did not benefit from hormonal therapy in high-risk ER-positive disease. HAGE+ and TILs were found to be independent predictors for pathological complete response to neoadjuvant-ACT; P<0.001. A statistically significant loss of HAGE expression following neoadjuvant-ACT was found (P=0.000001), and progression-free survival was worse in those patients who had HAGE+ residual disease (P=0.0003). Conclusions: This is the first report to show HAGE to be a potential prognostic marker and a predictor of response to ACT in patients with BC